Table 1.
Highlights of microarray results consisting of candidates with greater than twofold expression differences at P < 0.00001 (shaded boxes in Fig. 3)
| Type with higher expression | Accession no. | L | A | C | Diff. | P | Top match | Function | Tribolium castaneum match |
|---|---|---|---|---|---|---|---|---|---|
| RR | EW774177 | 452 | − | S | 2.04 | 11.30 | NP_000178 (S = 215, E = 9e-55) | Homogentisate 1,2-dioxygenase | XP_973513: PREDICTED: similar to CG4779-PA (S = 211, E = 1e-53) |
| CN497936 | 827 | − | C | 30.27 | 7.35 | None | |||
| EW761417 | 511 | − | C | 6.07 | 6.83 | None (no open reading frame) | |||
| CN497825 | 742 | − | C | 6.60 | 5.71 | XP_967924 (S = 125, E = 2e-27) | UDP-glucoronosyl and UDP-glucosyl transferase | XP_967924: PREDICTED: similar to CG18578-PA (S = 125, E = 2e-27) | |
| Contig1160* | 889 | − | C | 4.66 | 5.54 | ACI32832 (S = 230, E = 3e-73) | Beta-1,3-glucanase | XP_970010: PREDICTED: similar to beta-1,3-glucanase (S = 256, E = 2e-71) | |
| EW775372 | 333 | − | S | 14.18 | 5.16 | None | |||
| EW762256 | 434 | + | S | 16.61 | 5.11 | None | |||
| EW774489 | 547 | − | C | 10.51 | 4.59 | None | |||
| WT | EW762768 | 453 | − | S | 4.88 | 9.96 | ABP97089 (S = 57.8, E = 3e-07) | Antibacterial peptide | None |
| EW769719 | 633 | + | C | 2.30 | 8.22 | ABP97089 (S = 55.5, E = 1e-06), note only 200b match | Antibacterial peptide | None | |
| EW771833 | 573 | + | C | 5.92 | 8.13 | ABP97089 (S = 48.9, E = 3e-11; S = 42.0, E = 3e-11) | Antibacterial peptide | None | |
| EW768123 | 628 | + | S | 5.80 | 8.12 | ABP97089 (S = 76.3, E = 7e-13) | Antibacterial peptide | None | |
| EW763389 | 453 | + | C | 4.61 | 7.93 | None | |||
| EW770378 | 605 | − | S | 3.34 | 7.81 | ABP97089 (S = 91.3, E = 2e-17) | Antibacterial peptide | None | |
| EW765267 | 603 | + | C | 4.64 | 7.54 | ABP97089 (S = 73.9, E = 3e-12) | Antibacterial peptide | None | |
| EW772775 | 398 | + | C | 3.17 | 7.44 | AAK35160 (S = 77.0, E = 4e-13) | Defensin | XP_967194: PREDICTED: similar to CG1385-PA (S = 72.0, E = 1e-11) | |
| EW770481 | 211 | − | C | 2.88 | 6.73 | AAK35160 (S = 64.7, E = 2e-09) | Defensin | XP_967194: PREDICTED: similar to CG1385-PA (S = 54.3, E = 3e-06) | |
| EW767301 | 547 | + | S | 2.84 | 6.04 | ABP97089: (S = 59.3, E = 9e-08) | Antibacterial peptide | None | |
| EW761497 | 463 | + | S | 3.07 | 5.92 | None | |||
| EW769816 | 627 | + | C | 3.19 | 5.89 | ABP97089: (S = 91.3, E = 2e-17) | Antibacterial peptide | None | |
| EW764671 | 546 | + | S | 3.79 | 5.79 | None | |||
| EW766935 | 416 | + | C | 3.27 | 5.32 | AAW57774: S = 44.7, E = 0.002 | Parcxpwnx03 | None | |
| EW765002 | 343 | − | S | 2.53 | 5.28 | AAK35160 (S = 81.3, E = 2e-14) | Defensin | XP_967194: PREDICTED: similar to CG1385-PA (S = 54.3, E = 3e-06) | |
| EW767372 | 650 | + | C | 2.54 | 5.18 | ABP97089 (S = 56.2, E = 7e-07) | Antibacterial peptide | None | |
| EW774852 | 316 | − | S | 3.54 | 4.79 | NP_650064: CG5214 (S = 102, E = 7e-21) | Dihydrolipoyllysine-residue succinyltransferase activity | XP_971313: PREDICTED: similar to CG5214-PA (S = 97.8, E = 9e-19) |
RR, rotation-resistant; WT, wild-type; L, length of EST; A, presence of polyA tail; C, nontig (C) versus singleton (S); Diff., difference in expression between types as a fold change relative to the other behavioral type; P, significance of difference as −log10 of P-value.
Top match: top match in BLASTx search for known proteins in nonredundant database in GenBank. Cutoff was set at E < 10−4; scores (S) and expected (E) values are reported. Function: putative function ascertained from top match described above, species names of accession nos are as follows: ABP97089, Copris tripartitus (Coleoptera: Scarabeidae); AAK35160, Acalolepta luxuriosa (Coleoptera: Cerambycidae); ACI32832, Anthocharis cardamines (Lepidoptera: Pieridae); NP_000178, Homo sapiens; NP_650064, Drosophila melanogaster.
Contig1160 is composed of at least eight expressed sequence tag (EST), of which most of the consensus sequence in contained in CN497302.