Table 3.
Representative isolates from which translation elongation factor (EF-1α), mitochondrial small subunit (mtSSU) rDNA, nitrate reductase (NIR), and phosphate permease (PP) sequences were generated in this study to assess the phylogenetic relationships between Australian Fusarium oxysporum and F. oxysporum f. sp vasinfection (Fov) race 1–8 and between Australian F. oxysporum and those found elsewhere in the world
| Accession identifier | Pathogenicity on Gossypium hirsutum | Lineage | Subgroup/haplotype* | Origin, race or VCG group | EF-1α GenBank acc no. | mtSSU GenBank acc no. | NIR GenBank acc no. | PP GenBank acc no. |
|---|---|---|---|---|---|---|---|---|
| Accessions from Boggabilla site | ||||||||
| 7080 | Pathogenic | A | A-I/A02 | Field soil | DQ435339 | DQ435357 | –† | – |
| 251104 | Pathogenic | A | A-I/A05 | Diseased plant | DQ435340 | DQ435358 | – | – |
| 241117 | Pathogenic | A | A-II/A14 | Diseased plant | DQ435341 | DQ435359 | – | – |
| 7110 | Pathogenic | A | A-II/A16 | Field soil | DQ435342 | DQ435360 | – | – |
| 7094 | Nonpathogenic | A | A-I/A06 | Field soil | DQ435343 | DQ435361 | – | – |
| 7135 | Nonpathogenic | A | A-I/A08 | Field soil | DQ435344 | DQ435362 | – | – |
| 6521 | Nonpathogenic | A | A-I/A11 | Refuge soil | DQ435345 | DQ435363 | – | – |
| 7099 | Nonpathogenic | A | A-I/A12 | Field soil | DQ435436 | DQ435364 | – | – |
| 6543 | Nonpathogenic | A | A-I/A13 | Refuge soil | DQ435347 | DQ435365 | – | – |
| 7081 | Nonpathogenic | A | A-II/A19 | Field soil | DQ435348 | DQ435366 | – | – |
| 7108 | Nonpathogenic | B | /B26 | Field soil | DQ435349 | DQ435367 | – | – |
| 7070 | Nonpathogenic | E | /E32 | Field soil | DQ435350 | DQ435368 | – | – |
| Accessions from soil associated with wild Gossypium | ||||||||
| 2613 | Slightly pathogenic | A | A-I/A14 | Wild Gossypium soil | DQ435351 | DQ435369 | EU246622 | EU246656 |
| 3545 | Nonpathogenic | A | – | Wild Gossypium soil | EU246540 | EU246587 | EU246623 | EU246657 |
| 3546 | Slightly pathogenic | A | – | Wild Gossypium soil | EU246541 | EU246588 | EU246624 | EU246658 |
| 3547 | Slightly pathogenic | A | – | Wild Gossypium soil | EU246542 | EU246589 | EU246625 | EU246659 |
| 3549 | Nonpathogenic | A | A-I/A15 | Wild Gossypium soil | DQ435352 | DQ435370 | EU246626 | EU246660 |
| 3556 | Slightly pathogenic | A | – | Wild Gossypium soil | EU246543 | EU246590 | EU246627 | EU246661 |
| 3608 | Nonpathogenic | A | – | Wild Gossypium soil | EU246544 | EU246591 | EU246628 | EU246662 |
| 6510 | Nonpathogenic | A | – | Wild Gossypium soil | EU246545 | EU246592 | EU246629 | EU246663 |
| 6632 | Nonpathogenic | A | – | Wild Gossypium soil | EU246546 | EU246593 | EU246630 | EU246664 |
| 1517 | Slightly pathogenic | B | – | Wild Gossypium soil | EU246586 | EU246619 | – | – |
| 1537 | Nonpathogenic | B | – | Wild Gossypium soil | EU246585 | EU246620 | – | – |
| 2631 | Slightly pathogenic | E | – | Wild Gossypium soil | EU246562 | EU246597 | EU246634 | EU246668 |
| 3506 | Slightly pathogenic | E | – | Wild Gossypium soil | EU246563 | EU246598 | EU246635 | EU246669 |
| 3522 | Nonpathogenic | E | – | Wild Gossypium soil | EU246564 | EU246599 | EU246636 | EU246670 |
| 3544 | Nonpathogenic | E | – | Wild Gossypium soil | EU246565 | EU246621 | – | – |
| 3552 | Slightly pathogenic | E | – | Wild Gossypium soil | EU246566 | EU246600 | EU246637 | EU246671 |
| 4511 | Slightly pathogenic | E | – | Wild Gossypium soil | EU246567 | EU246601 | EU246638 | EU246672 |
| 4590 | Slightly pathogenic | E | – | Wild Gossypium soil | EU246568 | EU246602 | EU246639 | EU246673 |
| 6519 | Nonpathogenic | E | – | Wild Gossypium soil | EU246569 | EU246603 | EU246640 | EU246674 |
| Reference accessions obtained from public collections | ||||||||
| SC1‡ | Fov | – | – | Race 1 | EU246574 | EU246608 | EU246645 | EU246679 |
| IMI-141148‡ | Fov | – | – | Race 2 | EU246571 | EU246605 | EU246642 | EU246676 |
| IMI-338122‡ | Fov | – | – | Race 3 | EU246573 | EU246607 | EU246644 | EU246678 |
| IMI-141112‡ | Fov | – | – | Race 4 | EU246570 | EU246604 | EU246641 | EU246675 |
| IMI-325576‡ | Fov | – | – | Race 5 | EU246572 | EU246606 | EU246643 | EU246677 |
| ATCC-16611‡ | Fov | – | – | Race 6 | EU246549 | EU246596 | EU246633 | EU246667 |
| Ag6‡ | Fov | – | – | Race 7 | EU246547 | EU246594 | EU246631 | EU246665 |
| Ag85‡ | Fov | – | – | Race 8 | EU246548 | EU246595 | EU246632 | EU246666 |
| 24500§ | Fov | – | – | VCG 01111 | EU246575 | EU246609 | EU246646 | EU246680 |
| 24595§ | Fov | – | – | VCG 01111 | EU246576 | EU246610 | EU246647 | EU246681 |
| 041101 | Fov | – | – | VCG 01111 | EU246578 | EU246611 | EU246648 | EU246682 |
| 051101 | Fov | – | – | VCG 01111 | EU246579 | EU246612 | EU246649 | EU246683 |
| X1§ | Fov | – | – | VCG 01111 | EU246577 | EU246613 | EU246650 | EU246684 |
| 24492§ | Fov | – | – | VCG 01112 | EU246584 | EU246618 | EU246655 | EU246689 |
| 24597§ | Fov | – | – | VCG 01112 | EU246580 | EU246614 | EU246651 | EU246685 |
| 24598§ | Fov | – | – | VCG 01112 | EU246581 | EU246615 | EU246652 | EU246686 |
| 24646§ | Fov | – | – | VCG 01112 | EU246582 | EU246616 | EU246653 | EU246687 |
| B/96/02§ | Fov | – | – | VCG 01112 | EU246583 | EU246617 | EU246654 | EU246688 |
*
Identified based on results illustrated in Fig. 6.
†
Data not available.
‡
Provided in the form of DNA by Linda Smith (Queensland Department of Primary Industries, Indooroopilly, Australia).
§
Provided in culture by Natalie Moore, Linda Smith, and Wayne O'Neil (Queensland Department of Primary Industries, Indooroopilly, Australia).