Skip to main content
. 2011 Dec;92(Pt 12):2879–2888. doi: 10.1099/vir.0.037648-0

Fig. 4.

Fig. 4.

PB2-KO virus-based microneutralization assay. (a) Growth kinetics of CA04/PB2–Rluc monitored over 72 h. Wild-type AX4 and AX4/PB2 cells were infected with wild-type CA04 or CA04/PB2–Rluc virus at an m.o.i. of 0.001. Supernatants collected at the indicated time points were assayed for infectious virus by plaque assay in AX4/PB2 cells. (b) AX4/PB2 cells were infected with 100 p.f.u. CA04/PB2–Rluc that had been pre-mixed with serially diluted ferret serum samples in triplicate wells. Rluc activity in cells was measured using a Renilla luciferase assay system at 24 h p.i. The results from virus-infected cells were compared with those from cells that were infected with serum-untreated (−) virus. P values were calculated using Student’s t-test. *, P<0.05. RLU, Relative light units.