Table 1. Genetic differences between parental (WN/IC-P991 and WN-MX03) and point-mutant viruses generated.
Nucleotide numbers correspond to WNV NY99 (strain 382-99) genomic positions. Nucleotide positions are presented for 5′- and 3′UTR mutations and amino acid positions within respective genes are presented for amino acid changes. Amino acid substitutions are presented in upper case and nucleotide substitutions in lower case. Dashes represent the same nucleotide or amino acid identity compared with the WN/IC-P991 sequence.
| Parental/mutant recombinant virus | 5′UTR (nt 50) | 5′UTR (nt 71) | prM (aa 141) | E (aa 156) | NS4B (aa 245) | NS5 (aa 898) | 3′UTR (nt 10 828) | 3′UTR (nt 10 989) |
| WN/IC-P991 (NY99)* | a | a | I | S | I | T | t | g |
| WN/IC-5′ | g | g | – | – | – | – | – | – |
| WN/IC-prM | – | – | T | – | – | – | – | – |
| WN/IC-E | – | – | – | P | – | – | – | – |
| WN/IC-5′.prM.E | g | g | T | P | – | – | – | – |
| WN/IC-5′.E | g | g | – | P | – | – | – | – |
| WN/IC-prM.E | – | – | T | P | – | – | – | – |
| WN/IC-NS4B | – | – | – | – | V | – | – | – |
| WN/IC-E.NS4B | – | – | – | P | V | – | – | – |
| WN/IC-NS4B.NS5 | – | – | – | – | V | I | – | – |
| WN/IC-3′ | – | – | – | – | – | – | g | a |
| WN/IC-.E.3′ | – | – | – | P | – | – | g | a |
| WN/IC-5′.E.3′ | g | g | – | P | – | – | g | a |
| WN-MX03/IC-prM.E | g | g | – | – | V | I | g | a |
| WN-MX03/IC | g | g | T | P | V | I | g | a |
| TM171-03-pp1† | g | g | T | P | V | I | g | a |
GenBank accession no. AF196835 (Lanciotti et al., 1999). These substitutions represent two additional genetic differences between the WN/IC-P991 and WN-MX03 strains presented within this table.
Nucleotide and amino acid differences between the parental NY99 WNV (strain 382-99; synonymous with the exception of the abovementioned differences with WN/IC-P991) and the TM171-03-pp1 consensus sequence.