Not3/5 homologs from 12 Drosophila species. Clustal W [1, 2] was used to align sequences extracted from FlyBase. Amino acid (aa) color-coding is from Clustal W: red, small aliphatic, hydrophobic, and aromatics; blue, acidic; magenta, basic; green, hydroxyl, sulphydryl, amine, and glycine; grey, unusual aa. Symbols for aa conservation are from Clustal W: (asterisk ∗): positions with a single, fully conserved residue. (Colon :): conservation between groups of strongly similar properties-scoring >0.5 in the Gonnet PAM 250 matrix. (Period .): conservation between groups of weakly similar properties-scoring ≤0.5 in the Gonnet PAM 250 matrix.