Figure 6.

Inaccurate automated PRIA segmentation of necrotic tissue within teratomas. (a) Necrosis within a teratoma, characterized by amorphous, eosinophilic cellular debris mixed with nuclear debris, characteristic of dying cells. (b) Pseudo-color mark-up of image depicted in (a) following PRIA [Teratoma 1, Table 1]. Despite a training class encompassing cell debris (orange), the region of necrosis was inaccurately segmented as nervous tissue (yellow) and muscle (green), with flecks of cartilage (blue). The algorithm more accurately mapped the congested vasculature present (purple). Bars = 50 μm