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(A–B) The GFP and 111L-GFP expression in pEGFP-N3- and p111L-GFP-injected embryos were shown. (C–D) Empty vector pEGFP-N3-injected embryo did not show any phenotypic abnormality, while the yolk sac was not completely developed in 111L-GFP overexpressing embryo. (E–F) Hematoxylin-eosin staining assay was performed in GFP- and 111L-GFP-overexpressing embryo.
