Table 1. Summary of primers used in this study.
Primers | Primer sequence (5′-3′) |
Overexpression assay | |
111L-GFP-F | CGGAATTCATGGAACTGTGTCAGCCC |
111L-GFP-R | CGGGATCCGAGATCGCACACGTGTA |
RFP-111L-F | GGGGTACCATGGAACTGTGTCAGCC |
RFP-111L-R | CGGGATCCGAGATCGCACACGTG |
111-RNA-F | ATGGACTACAAAGACGATGACGACAAGATGGAACTGTGTCAGCCCAACAAC |
111-RNA-R | CTAGAGATCGCACACGTGTACCTTGAT |
GST Pulldown assay | |
GST-111L-F | CGGGATCCATGGAACTGTGTCAGCCC |
GST-111L-R | CGGAATTCGAGATCGCACACGTGTA |
MYC-TRADD-F | GGGGTACCAATGGACAGTATAGACACAAAGAGG |
MYC-TRADD-R | CGGGATCCTTAATCTCGTGGCTGGAT |
RT-qPCR assay | |
β-actin-QF | ATGCCCCTCGTGCTGTTTTC |
β-actin-QR | GCCTCATCTCCCACATAG GA |
caspase 8-QF | AGACCAGGAACAAGGAGGCAGACT |
caspase 8-QR | CTGTAGTAATTGTGCCAGCCGAAGAG |
ISH riboprobe synthesis | |
caspase 8-PF | CGGAATTCCTGACAAGCGGTGATGTGGACC |
caspase 8-PR | GGGGTACCCATATCAGTGCCTGTTCGTTTGAGC |
caspase 3-PF | ATGCAGGTTGATGCCAAGCCT |
caspase 3-PR | TGAACAGACTAGTTAAAGACTTGAGATCCAC |
Morpholino assay | |
MO-caspase 8-F | CTGACAAGCGGTGATGTGGA |
MO-caspase 8-R | GCCCAAGCCTCTGTTGTTTT |
MO-β-actin-F | GACGACCCAGACATCAGGGAGTG |
MO-β-actin-R | TGGAGTTGAAGGTGGTCTCGTGGA |
The underlined letters is the restriction endonuclease cutting site.