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. 2012 May 15;7(5):e37001. doi: 10.1371/journal.pone.0037001

Table 1. Summary of primers used in this study.

Primers Primer sequence (5′-3′)
Overexpression assay
111L-GFP-F CGGAATTCATGGAACTGTGTCAGCCC
111L-GFP-R CGGGATCCGAGATCGCACACGTGTA
RFP-111L-F GGGGTACCATGGAACTGTGTCAGCC
RFP-111L-R CGGGATCCGAGATCGCACACGTG
111-RNA-F ATGGACTACAAAGACGATGACGACAAGATGGAACTGTGTCAGCCCAACAAC
111-RNA-R CTAGAGATCGCACACGTGTACCTTGAT
GST Pulldown assay
GST-111L-F CGGGATCCATGGAACTGTGTCAGCCC
GST-111L-R CGGAATTCGAGATCGCACACGTGTA
MYC-TRADD-F GGGGTACCAATGGACAGTATAGACACAAAGAGG
MYC-TRADD-R CGGGATCCTTAATCTCGTGGCTGGAT
RT-qPCR assay
β-actin-QF ATGCCCCTCGTGCTGTTTTC
β-actin-QR GCCTCATCTCCCACATAG GA
caspase 8-QF AGACCAGGAACAAGGAGGCAGACT
caspase 8-QR CTGTAGTAATTGTGCCAGCCGAAGAG
ISH riboprobe synthesis
caspase 8-PF CGGAATTCCTGACAAGCGGTGATGTGGACC
caspase 8-PR GGGGTACCCATATCAGTGCCTGTTCGTTTGAGC
caspase 3-PF ATGCAGGTTGATGCCAAGCCT
caspase 3-PR TGAACAGACTAGTTAAAGACTTGAGATCCAC
Morpholino assay
MO-caspase 8-F CTGACAAGCGGTGATGTGGA
MO-caspase 8-R GCCCAAGCCTCTGTTGTTTT
MO-β-actin-F GACGACCCAGACATCAGGGAGTG
MO-β-actin-R TGGAGTTGAAGGTGGTCTCGTGGA

The underlined letters is the restriction endonuclease cutting site.