Figure 4. Neural differentiation of TR−/S4+ cells.

TR−/S4+ cells were further differentiated for in N2B27 medium supplemented with noggin for 1–2 weeks, then without noggin. (A) Phase-contrast images of further culture of TR−/S4+ in neural differentiation media for 3–4 weeks. (B–F) Immunostaining with the indicated antibodies at different time points: nestin and Sox1 (3–4 weeks), MAP2 and β-tublin III (6 weeks) and GFAP (15 weeks). Scale bar represent 100 µm.