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. 2012 May 15;7(5):e37129. doi: 10.1371/journal.pone.0037129

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Noisa et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Images of cell aggregates at day 3 and 7 of differentiation, respectively. EB-like structures are visible by day 7 (arrows). Scale bar = 100 µm. (B) Images of cell aggregates cultured for a further 7 days after disassociation. Scale bar = 100 µm. (C) qRT-PCR analysis of markers in hESCs, TR−/S4+ and their differentiated progeny (TR−/S4+ EB) two weeks after initiation of differentiation (1 week in suspension and 1 week after dissociation onto adherent dish). (D) Immunostaining with indicated antibodies on differentiated progeny of TR−/S4+ cells 1 week after dissociation onto coverslip. Scale bar = 50 µm.