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. 2012 May 15;7(5):e37202. doi: 10.1371/journal.pone.0037202

Figure 4. TGF-β1−producing mast cells and IL-17−producing γδ T cells in tolerogenic liver allografts.

Figure 4

Cryosections of naïve and OLT livers at tolerogenic phase (>60 days) were immunoprobed with goat polyclonal Ab against MCP1, mouse monoclonal Ab against γδ TCR, and rabbit polyclonal Ab against TGF-β1 or IL-17 followed by incubation with Alexa Fluor® 488−conjugated donkey anti-goat or mouse IgG or HyLite Fluor™ 594−conjugated goat anti-rabbit IgG. Hoechst 33342 (specific to nucleus) was used for counterstaining. Data are representative of six individual liver sections (60× magnification). A higher magnification (210×) was used to show the colocalization of TGF-β1/MCP1 and IL-17/TCR γδ chain in tolerogenic liver allografts (upper right column).