Figure 7. Induction of liver regeneration by BMMCs.
Primary hepatocytes (2×105 cells) were co-cultured with syngeneic BMMCs (0, 2 and 4×105 cells) for 72 hrs. After washing twice with PBS, new culture medium was added together with MTT reagents, cultured for 4 hrs and the absorbance (595 nm) was measured after color development. Percent survival of hepatocytes relative to control (hepatocytes∶mast cell ratio = 1∶0) is represented as the mean of six independent culture wells ± SD. ** Significantly different compared with control (P<0.01).