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. 2012 May 15;7(5):e36301. doi: 10.1371/journal.pone.0036301

Figure 3. MYOC mutants’ top-changers contained numerous human trabecular meshwork relevant genes.

Figure 3

Adenoviral vectors carrying four MYOC mutations cassettes and no transgene (Ad5.CMV-Null) were infected on primary human trabecular meshwork cell line HTM-72 to overexpress MYOC mutant proteins. The expression of genes in the mutant-treated cells was compared with that of the cells treated with the empty virus, using Affymetrix GeneChips (n = 15). Non-redundant gene lists from the cutoff FC value of ≥ and ≤ 1.5 of each mutant were screened for trabecular meshwork relevant (TMR) genes. Each selected TMR gene was manually cross-checked to identify its expression in the other three mutants. A: twenty selected upregulated TMR genes in Q368X, R342K, D380N and K423E. B: twenty selected downregulated genes in Q368X, R342K, D380N and K423E.