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. 2012 May 15;7(5):e35283. doi: 10.1371/journal.pone.0035283

Figure 7. Smad-4 gene and protein expressions of Lipo-siRNA-Smad-4 transfected PASMCs.

Figure 7

(A) QRT-PCR demonstrated that the lowest Smad-4 gene expression was achieved when 120 nM siRNA-Smad-4 was used to transfect 1×105 PASMCs. (B) Abolishment of Smad-4 protein expression did not affect PTEN protein expression. Qantification of PTEN (C) and pAKT (D) protein expression after normalized to GAPDH (consider as 100%). (E) A non-coding siRNA was used as a negative control to determine the specificity of siRNA-Smad-4 targeting. (Lipo = lipofetamine-2000. *: vs lipo only, p<0.05; &: vs GM only, p<0.05).