Table 1. RT-PCR primers and annealing temperature.
GAPDH60°C, 90 bp, | forward | 5' AGCCACATCGCTCAGACAC 3' |
reverse | 5' TAAAAGCAGCCCTGGTGAC 3' | |
PTEN60°C, 155 bp | forward | 5' TCACCAACTGAAGTGGCTAAAGA 3' |
reverse | 5' CTCCATTCCCCTAACCCGA 3' | |
AKT-158°C, 125 bp | forward | 5' TAACCTTTCCGCTGTCGC 3' |
reverse | 5' ATGTTGTAAAAAAACGCCG 3' | |
AKT-258°C, 127 bp | forward | 5' GGTCGCCAACAGCCTCAA 3' |
reverse | 5' CACTTTAGCCCGTGCCTTG 3' | |
Smad-460°C, 128 bp | forward | 5' CTTCAGGGGCTTCTAAAACAG 3' |
reverse | 5' TATCAGAGAGGGAAGAGACCAG 3' |