Figure 4. CsA inhibited the influenza virus replication at post-transcriptional level.

293T/CypA− and 293T/CypA+ cell lines were infected with influenza A/WSN/33 (MOI = 1) in the absence or presence of CsA (5 µg/ml). RNA of the infected cells at 2 h, 4 h and 8 h p.i. was extracted and assayed using the specific primers for influenza RNA by RT-PCR. The M1 mRNA level (A and B), vRNA level (C and D) and cRNA level (E and F) of the both cell lines were quantified by real-time PCR analysis using the specific primer for M1. The mRNA of GAPDH was kept as an internal control. Data are means ± SD of three separate experiments.