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GST pull-down assays between GST-CypA and M1, GST-CypB and M1 were performed in the absence (lane 3, lane 7) or presence (lanes 4, 5, 8, 9) of CsA. The concentrations of CsA in lanes 4, 5, 8, 9 are 2.5, 5, 2.5, 5 µg/ml, respectively. Coomasie brilliant blue (CBB) staining patterns for the pulled-down proteins are shown in the bottom panel.
