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. 2012 May 15;7(5):e36113. doi: 10.1371/journal.pone.0036113

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Chu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Wild-type A/California/04/09 virus and recombinant virus possessing the K480R mutation were used for infection of human Calu-3 cells (MOI = 0.001). Supernatants were collected at specified time points following infection, and virus titers were quantified by plaque assays in MDCK cells. Results shown are averages of three plaque assays.