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. 2012 May 15;7(5):e36113. doi: 10.1371/journal.pone.0036113

Table 3. Mutations in the conserved motifs I – IV of the PB1 protein and their effects on replicative ability in human and chicken cells.

Motif Mutation(s) Mutant BHG polymerase activity (%)1 Virus(es) possessing the respective mutation(s)
Human cells Chicken cells
T303I 27.2 53.9 A/chicken/Taiwan/G2/87 (H6N1)A/duck/HK/412/78 (H4N2)
I T303S 78.9 88.8 A/Guangxi/1/05 (H5N1)
N306T 2.4 0.8 A/Thailand/271/05 (H1N1)
II M409T 0 0.2 A/swine/Nordkirchen/IDT1993/03 (H3N2)
D439E 49.0 34.2 A/bar-headed goose/Qinghai/1/05 (H5N1)A/whooper swan/Qinghai/01/05 (H5N1)
Q442L 19.2 6.2 A/duck/Vietnam/8/05 (H5N1)A/duck/Vietnam/GZ-8/05 (H5N1)A/Hong Kong/483/97 (H5N1)
Q442R/D445E/F447L 0 0.3 A/duck/Hubei/wq/03 (H5N1)
S444P 0 0.4 A/chicken/Anhui/1089/07 (H5N1)
III D445G 0 0.5 A/mallard/Yan chen/05 (H4N6)
D445S/D446S 0 0.3 A/swine/Doetlingen/IDT4735/2005 (H1N2)
D445C/F447V/L449I 0 0.3 A/chicken/Shantou/1126/01 (H9N2)
D446N 0 0.3 A/chicken/Guangdong/5/97 (H9N2)A/chicken/Guangdong/10/00 (H9N2)A/chicken/Jiangsu/1/00 (H9N2)
A448V 40.8 96.9 A/swine/Shanghai/1/07 (H1N2)A/swine/North Carolina/93523/01 (H1N2)
I475V 67.6 73.1 A/Indonesia/CDC292N/05 (H5N1)A/Queensland/60/05 (H3N2)A/chicken/Taiwan/2838N/00 (H6N1)
N476Y 0 0.3 A/swine/Anhui/ca/04 (H5N1)
N476D 0 0.3 A/Indonesia/286H/06 (H5N1)A/chicken/Taiwan/G2/87 (H6N1)
IV N476L/M477T 0 0.4 A/great black-headed gull/Qinghai/2/05 (H5N1)
N476T/S478T 0 0.3 Four 2005 H5N1 (Qinghai-Lake) isolates2
S478T 0 0.3 Four 2005 H5N1 (Qinghai-Lake) isolates2
S478R 0 0.2 A/swine/Guangxi/wz/04 (H5N1)
K480R 117.2 113.1 Several pandemic (2009) H1N1 viruses; several swine viruses
K481E 0 0.3 A/duck/Hunan/3340/06 (H5N1)
K481T 0 0.2 A/chicken/Hong Kong/873.3/01 (H5N1)
K481N 0 0.3 A/turkey/Israel/1567/04 (H9N2)
Y483H 0.5 0.3 A/swine/Korea/S83/04 (H9N2)
1

The indicated mutations were introduced into the PB1 protein of A/bar-headed goose/Qinghai/1/2005 (H5N1; BHG) virus. Polymerase activity in human 293T and avian DF-1 cells is indicated as percentage relative to wild-type PB1 activity.

2

For some of these isolates, different sequences are reported in the Los Alamos National Laboratory and NIH NCBI databases.