Table 2. Digital differential expression analysis of c-Kit, Tyrp1, Tyr and Tyrp2 genes between black and white feather bulbs by RNA-Seq.

Gene	Raw-B-1	Raw-W-1	TPM-B-1	TPM-W-1	Log2ratio(W-1/B-1)	P-Value			FDR
c-Kit	160	16	32.74	3.29	−3.31489	3.44E-31				5.00E-29
Tyrp1	3250	0	664.89	0.01	−16.021			0		0
Tyr	184	0	37.65	0.01	−11.8784		5.96E-56			3.48E-54
Tyrp2	0	0

Note: Raw-B-1, Raw data of black feather bulb expression; Raw-W-1, Raw data of white feather bulb expression; TPM-B-1, Normalized expression level of genes in black plumage feather bulb library; TPM-W-1, Normalized expression level of genes in white plumage feather bulb library; Log₂ ratio(W-1/B-1), log₂(multiples of differentially expressed genes); P-value corresponds to differential gene expression test; FDR (False Discovery Rate) is used to determine the threshold P-value in multiple tests and analyses by manipulating the FDR value [27].