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. 2012 May 15;7(5):e36873. doi: 10.1371/journal.pone.0036873

Figure 3. Secretion of human tau is reduced at low temperature and is not prevented by BFA treatment.

Figure 3

(A) The amount of GFP-tau in M (lower arrow) was reduced at low temperature (18°C and 4°C) whereas the expression of tau was not affected (upper arrow). (B) Human tau fused to a Flag tag was also secreted by Hela cells. Secreted Flag-tau was cleaved as noted for GFP-tau (upper and lower arrows). The secretion of Flag-tau (lower arrow) but not its expression (upper arrow) was also impaired at low temperature. (C and D) The secretion of both GFP-tau and Flag-tau was not affected by BFA. Control cells (Ctrl) were treated with DMSO, the vehicle of BFA. BFA treatment was tested at least in three sets of experiments. E) The amount of wild-type tau in M was not decreased after ultracentrifugation to remove microvesicles/exosomes.