Figure 8. The effect of leptin on osteogenesis of MSCs from a normal control (22y, male) and an AIS patient (18y, male, Cobb angle = 88°).

Cells from the normal control were induced along the osteogenic differentiation with human recombine leptin protein at concentration of 0 µg/mL (a), 0.015 µg/mL (b) and 0.6 µg/mL (c). MSCs from the AIS patient were also treated under the same condition that contained leptin of 0 µg/mL (d), 0.015 µg/mL (e) and 0.6 µg/mL (f). At day 21, the cells were subjected to Alizarin Red S staining. Scale bar = 500 µm.