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. 2012 May 14;197(4):535–551. doi: 10.1083/jcb.201110034

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Figure 5. — Hydrogen peroxide treatment enlarges mitochondria in Drp1KO MEFs. (A) Wild-type and Drp1KO MEFs were incubated in the presence and absence of 50 µM hydrogen peroxide for 1, 2, and 3 h. Cells were then fixed and subjected to immunofluorescence with antibodies to PDH and Tom20. Images at 2 h are shown. (B) Time course of mitochondrial enlargement. Cells that contained enlarged mitochondria were scored. Values represent the mean ± SEM (n = 3). More than 100 cells were examined in each experiment.