Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 May 14;197(4):535–551. doi: 10.1083/jcb.201110034

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — Purkinje cells die after Drp1 loss in vitro. (A) Cerebellar neurons were isolated from P0 Drp1^flox/flox mice and cultured for 20 d. When cells were plated (d 0), lentiviruses carrying Cre recombinase and GFP in tandem (Drp1KO) or GFP alone (control) were added to the culture. At d 10 and 20, cells were fixed and analyzed by immunofluorescence using anti-calbindin antibodies. (B) Quantification of the number of Purkinje cells and total cells. To count the number of Purkinje cells and total cells, calbindin staining and DAPI staining were used, respectively. Cell numbers were normalized to those of control cells at d 10 in each experiment. Values represent the mean ± SEM (n = 3). (C) Cultured cerebellar neurons were infected with lentiviruses carrying the Cre recombinase (Drp1KO) and the myc epitope (control). Mitochondria were visualized with immunofluorescence using antibodies to PDH, calbindin, and Tom20 in control and Drp1KO Purkinje cells at d 10. Dendrites and axons are indicated by arrowheads and arrows, respectively.