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. 2012 May 14;197(4):535–551. doi: 10.1083/jcb.201110034

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Figure 7. — Mitochondria accumulate autophagy markers in cultured Drp1KO Purkinje cells. (A–E) Cultured cerebellar neurons were infected with lentiviruses carrying the Cre recombinase (Drp1KO) and the myc epitope (control) at d 0 and cultured for 20 d. Immunofluorescence was performed using antibodies to calbindin to identify Purkinje cells, PDH, and Tom20 to label mitochondria, as well as antibodies to ubiquitin (B), 4-hydroxynonenal (HNE) (C), LC3 (D), and p62/SQSTM1 (E). The soma of Purkinje cells are shown. Nuclei (N) were stained due to ubiquitination of histones (Muratani and Tansey, 2003) in B. (F) Quantification of the number of Purkinje cells containing mitochondria with positive staining for the indicated marker. Values represent the mean ± SE.