Figure 2.

Effect of transgene induction on albuminuria. In panel A and B, albuminuria was measured after 2 weeks of treatment in both N19Rho “double” TG mice and V14Rho “double” TG mice as well as controls (“single” TG and non-TG mice). Treatment with doxycycline significantly increased albuminuria in V14Rho (panel A) and N19Rho (panel B) “double” TG mice compared to “double” TG mice at baseline or compared to controls at either baseline or after treatment with doxycycline. In panels C and D, mice were treated for 6 weeks with doxycycline and albuminuria was measured at the 2, 4 and 6-week time points. In V14Rho “double” TG mice (panel C), albuminuria was increased during doxycycline treatment compared to controls and tended to increase over time. At the 6-week time point, albuminuria was significantly increased compared to the 2-week time point in V14Rho “double” TG animals. Albuminuria was also increased in N19Rho “double” TG mice compared to controls after doxycycline treatment at all time points examined, but tended to remain relatively stable over the treatment period. Eight to 16 mice were studied per group. ‡P<0.05 vs “double” TG at baseline, *P<0.01 vs “double” TG at baseline, §P<0.05 vs controls after treatment with doxycycline, fP<0.01 vs controls after treatment with doxycycline, †P<0.001 vs controls after treatment with doxycycline, ¶P<0.01 vs “double” TG at 2 weeks