Figure 5. CD40 levels on migrating DCs and not DC migration is impaired in TCRδ-/- mice.
(A) C57 and TCRδ-/- mice, grafted with syngeneic skin, or non-grafted were painted with FITC on graft site or a corresponding site on non-grafted mice. 4 days later, DLNs were analysed for FITC+ migrating DC as a percentage of all DC (P= N.S) (B) Graft DLNs of C57 (n=4) and TCRδ-/- (n=4) mice grafted with congenic Ly5.1+ skin grafts were analysed 4 days later for Ly5.1+ migrating DC as a percentage of all DC (P= N.S) (C) CD40 expression on migrating DC in skin DLNs cells from grafted or non-grafted C57 and TCRδ-/- mice (**: P <0.01; unpaired t test). Histogram shows representative isotype and CD40 staining on migrating DCs.