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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Anal Chem. 2012 May 2;84(10):4618–4621. doi: 10.1021/ac300496q

Figure 1.

Figure 1

Sequence of devices for adhesion-based microfluidic separation of cells against multiple surface markers. Following capture and release from device (i), cells expressing CD34 enter device (ii) where a calcium chloride solution is co-injected to neutralize the EDTA present in the cell suspension. Device (iii) mixes the calcium chloride solution and cell suspension. Device (iv) captures cells against the second marker, Flk1, which are eluted out by EDTA solution introduced at the inlet to device (i).