Fig. 3.

Mean relative CK activities in sera determined continuously in the same mdx mice given EGCG a sc (n = 4–7 for each experimental point) or b orally in the diet (n = 4–10 for each experimental point). The serum CK levels at each age point of control WT mice treated with saline (n = 7 or 8) or a control diet (n = 4–6) were normalized by dividing the activities by the mean CK activities in 3-week-old mdx mice (11,300 U/l) before EGCG treatment. When compared with the activities in corresponding mdx controls, the mean relative CK activities of mdx mice were reduced significantly by 57 ± 7% (n = 7) after 5-week sc administration with the lower dose of EGCG and by 45 ± 8% (n = 9) after 4-week oral administration of EGCG. Significance levels denoted as ***P < 0.001; **0.001 ≤ P≤ 0.01; *0.01 < P ≤ 0.05 are for the comparison to corresponding mdx controls