Dvl protein distribution in blastocyst on embryonic day 4. A. Morphology of freshly flushed E4.0 expanded blastocyst (a) and E4.5 implanting blastocysts (b and c) B. a-c, E4.25 blastocysts, confocal middle section. d-f, E4.5 blastocysts, confocal middle section. g-i, E4.5 blastocysts, 3-D projection. Dvls, red. DNA, blue. MTE, mural trophectoderm. ICM, inner cell mass. Arrow heads indicate the specific accumulation of Dvl2 on the cell membrane and Dvl3 in the cytoplasm of the ICM cells. C. Enlargement of Dvl1, 2 and 3 subcellular localization in one blastomere. Arrow heads hightlight the cyplamic puncta and membrane concentration of Dvl proteins.