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. Author manuscript; available in PMC: 2012 May 16.
Published in final edited form as: Dev Biol. 2006 Aug 22;302(1):40–49. doi: 10.1016/j.ydbio.2006.08.036

Figure 3.

Figure 3

(A) Western blot analysis showed increased levels of dephosphorylated β-catenin and Dvl3 proteins in E4.5 blastocysts. 120 embryos per lane. The stage is as listed. Arrows indicate bands for β-catenin (100 kDa) and Dvl3 (93 kDa). (B) lacZ staining of F1 E4.0 (a), BAT-gal E4.0 (b), E5.5 (c) and E6.25 (d) embryos, positive signal was only detected in the extraembryonic visceral endoderm at E5.5 and the site where future primitive streak is going to form at E6.25. (C) RT-PCR analysis of Kaiso and p120catenin expression. The identities of all the PCR products were confirmed by DNA sequencing.