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Overexpression of Dvl1-3-GFP did not activate canonical Wnt pathway in blastocysts. A. mRNAs encoding GFP (a) or Dvl1-3-GFP (b, c and d respectively) were injected into one blastomere of 4-cell embryos from BAT-gal Wnt reporter mice. B. The injected embryos were cultured to blastocyst stage and staining for lacZ. No signal was detected in all cases even after one-week incubation with the substrate.
