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. Author manuscript; available in PMC: 2012 May 16.
Published in final edited form as: Dev Biol. 2006 Aug 22;302(1):40–49. doi: 10.1016/j.ydbio.2006.08.036

Figure 6.

Figure 6

Overexpression of Dvl-GFPs does not affect compaction. GFP (A) or Dvl1, 2 ,3-GFP (B) mRNA were microinjected into one blastomere of late 4-cell or early 8-cell embryos. They were cultured for further 6 hours when non-injected sibling embryos were compacted. Embryos were then briefly exposed to Rhodamine conjugated Concanavalin A and subjected to confocal live imaging. Concanavalin A staining is shown in red, Dvl-GFPs is in green and transmitted light channel is in blue. Arrow head (A) and arrows (B) indicate the apical staining of microvilli.