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. Author manuscript; available in PMC: 2012 May 16.
Published in final edited form as: Dev Biol. 2006 Aug 22;302(1):40–49. doi: 10.1016/j.ydbio.2006.08.036

Figure 7.

Figure 7

Overexpression of Dvl-GFPs perturbs cell flattening and adhesion in blastocyst. Late 4-cell or early 8-cell embryos were injected in one blastomere with GFP or Dvl-GFPs and cultured for 24 hours (by which time the uninjected sibling embryos started to form blastocyst). Embryos were then subject to multi-channel, multi-section and time-lapse filming for 8 hours to record the process of blastocyst formation. Both GFP channel and brightfield channel images were shown. Time is as indicated. (A) Embryos injected with GFP formed normal blastocyst. (B and C) Blastomeres expressing Dvl-GFPs are more rounded. One blastomere failed to flatten (B, arrow head), another was excluded into the blastocoel (B, arrow). In C, the progeny of Dvl-GFP expressing blastomeres became scattered.