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. 2012 Apr 10;12:38. doi: 10.1186/1472-6882-12-38

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Copyright ©2012 Castañeda et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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P2Et fraction thin layer chromatographic profile. (A) Solvent I: petroleum benzene - ethyl acetate - formic acid (40:60:1). (B) Solvent II: chloroform - ethyl acetate - acetic acid (50:50:1). (C) Solvent III: Toluene - acetonitrile - formic acid (70:30:1). Lane 1: P2Et fraction basic hydrolysis products. Lane 2: S2Et fraction basic hydrolysis products. TLC visualization with UV, 254 nm, ferric chloride (FeCl₃) and vanillin/sulfuric acid (VS) sprays. Gallic acid is a positive control. (D) Gallic acid Rf values in different solvent systems. (E) HPLC chromatogram at 263 nm. (F) MS spectrum positive mode. (G) MS spectrum negative mode.