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. 2012 Mar 23;12:42. doi: 10.1186/1471-2229-12-42

Figure 1.

Figure 1

Validation of fifteen selected tentative PvRGLs by RT-PCR. Confirmation of absence of DNA contamination is shown in lanes 1-4 where RT-PCR amplification was carried out with primers designed from contig11286 in lanes with genomic DNA, leaf cDNA, leaf cDNA control (no reverse transcriptase added to reverse transcription reaction), and water as template to check DNA contamination. In lanes 5-18, RT-PCR products derived by amplification from an additional 14 common bean RGLs using leaf cDNA as a template are shown. M: 50 bp ladder.