Table 3.
Primer sequences and restriction enzymes for the RGL-tagged CAPS markers
| PvRGL | Forward primer | Reverse primer | Restriction enzymes* |
|---|---|---|---|
| PvRGL016 | TGCACCATGACTGTTCTCCACC | AATACCACCTCCAGCCCAAGA | HindIII/G |
| PvRGL039 | CTACTCCCTCAAAGGAATCACC | TGAGAAAGTTTTTGCTCAATGG | DraI/B, A |
| PvRGL040 | TCTTAAAGGACGCACCTACACG | ATTGGCCTTCAAGACGGTTAT | SnaBI/G |
| PvRGL041 | GCCTCCGTTTGAAAGTTGCTC | CACGAAATATCTTACGTTTGTTTGC | AgeI/G |
| PvRGL049 | GAACAATGTTAGTAGTACTTCGCC | GGCTTCCTCTTGAGAGTACTTTG | PflMI/S, O, G, B, P |
| PvRGL050 | CACATTAGTAGCCTTTATGTCCC | CTTCGGTCCTGTCTACAAGGT | HindIII/G |
| PvRGL065 | CGCTTAGGAGTTGGTAGAGTAACAC | TCAAGGAAACACCGACAGAATG | NsiI/S, O, B, P, A |
| PvRGL073 | CCAAAATCAGAAACTTTGGCATG | AGAGCCCTGGGCAGGAAAGAC | AflII/G |
| PvRGL093 | TGGGTTACTGTTGTGACGATGT | TATGTGCCCATGATCCTGGTAG | DraI/O, P |
| PvRGL099 | CTTCGCCATCTCCTTGTCTATC | GCCCTATGCTCAGCCTTTCTAG | BglII/B, G |
| PvRGL102 | TGAAGCCCTTATGCACAGGTCC | TCATCATCGCCAAACCCCAAC | XmnI/S, O, P |
| PvRGL153 | AGTGATTTCCATTTATACGAGG | AAAAGCCAAGTAACATTCTTCT | SphI/O |
| PvRGL155 | GAACCAGCAATTTGTTACCGAG | TAGTTGTTGAGCAACCTTTAGC | NdeI/O, B, P |
| PvRGL173 | GGATCATTGCCTGTATTCGAGG | TGGCACTGTCCCTGATAAACTG | EcoRI/G, B, A |
| PvRGL246 | CAGCCACCTTGGGTTGGTAATC | GACGCAGTTGCTAAATGGACACC | HindIII/S, O, P |
| PvRGL264 | GTAACTCGTGTCCTCCTCATCT | GATACTTGAGAAGGTAAAGGCTG | SpeI/B |
| PvRGL297 | TTGGAGCAATGAGAAAATAGGGG | TCCAGCAGAAGCCCTGTGATG | SpeI/G |
| PvRGL302 | CTCTTAAAGGACGCACCTACAC | TATTGGCCTTCAAGACGGTTAT | SnaBI/G |
| PvRGL308 | TAAGATGTGTCAATAAATATGCTG | CAGGAAGTTGCTGTAAAGAAGT | BspMI/G |
*The genotypes shown in this column can be digested by the corresponding restriction enzyme