Table 2.
Intra- and interassay variability of the SYBR green real-time PCR method.
| Intra- and interassay variability | ||||
|---|---|---|---|---|
| Samples | Replicate (and assay) numbers | Mean (SD) | log10 mean (SD) | log10 CV% |
|
| ||||
| Intraassay variability | ||||
| A1 | 3 (1) | 1,86E + 06 (2,35E + 05) | 6,27 (0,05) | 0,84 |
| A2 | 3 (1) | 2,03E + 05 (1,77E + 04) | 5,31 (0,04) | 0,71 |
| A3 | 3 (1) | 2,00E + 04 (8,96E + 02) | 4,30 (0,02) | 0,45 |
| A4 | 3 (1) | 2,26E + 03 (2,53E + 02) | 3,35 (0,05) | 1,41 |
| A5 | 3 (1) | 2,09E + 02 (8,22) | 2,32 (0,02) | 0,74 |
| A6 | 3 (1) | 1,98E + 01 (5,29) | 1,28 (0,13) | 9,99 |
| A7 | 3 (1) | 2,09E + 00 (0,44) | 0,31 (0,09) | 28,58 |
| B1 | 3 (1) | 5,79E + 02 (4,65E + 01) | 2,76 (0,03) | 1,27 |
| B2 | 3 (1) | 2,00E + 00 (0,10) | 0,30 (0,02) | 7,56 |
| B3 | 3 (1) | 4,01E + 00 (0,74) | 0,60 (0,08) | 13,68 |
|
| ||||
| Interassay variability | ||||
| B1 | 3 (3) | 6,08E + 02 (5,58E + 01) | 2,78 (0,04) | 1,38 |
| B2 | 3 (3) | 2,06E + 00 (0,34) | 0,29 (0,07) | 23,97 |
| B3 | 3 (3) | 3,11E + 00 (0,82) | 0,46 (0,13) | 28,79 |
Successive 10-fold dilutions of recombinant plasmid: A1 (2 × 106), A2 (2 × 105), A3 (2 × 104), A4 (2 × 103), A5 (2 × 102), A6 (2 × 101), and A7 (2 × 100).
Three bat samples with different viral concentrations: B1 (102), B2 and B3 (100).
SD: standard deviation.
CV: coefficient of variation.