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. Author manuscript; available in PMC: 2012 May 16.
Published in final edited form as: Nature. 2011 Aug 18;476(7360):346–350. doi: 10.1038/nature10350

Figure 3. Cell lines with elevated PHGDH expression have increased serine biosynthetic pathway activity and are sensitive to PHGDH suppression.

Figure 3

a, Serine biosynthesis pathway (SBP). b–d, Serine production by SBP in (b) indicated breast cell lines, (c) after PHGDH suppression by siRNA, and (d) MCF-10A cells expressing PHGDH or PSPH cDNAs with associated immunoblots. e–f, Immunoblots of indicated proteins (e) for indicated cell lines expressing control shRNA (GFP) or shRNAs against PHGDH (PHGDH_1 and PHGDH_2). Relative proliferation (f) of cells transduced with shRNA constructs after seven days. g, Images showing cellular morphology of MDA-MB-468 at day seven of (f). h, Tumour growth of MDA-MB-468 cells expressing doxycycline inducible control shRNA (GFP) or shRNA against PHGDH (shPHGDH_2) in mice fed doxycycline (Dox, 2mg/kg, green lines, n=5) or normal (blue lines, n=4) diet after initial tumour palpation (Day 0). Immunoblots of PHGDH or RPS6 (S6) shown for cells in vitro. Asterisks indicate p < 0.05 relative to control. Error bars for metabolite measurements (n=4) and tumour size indicate SEM and for cell number indicate SD (n=3).