A flow diagram of the cross design. Families are pure wild (W; 0% domesticated alleles), second-generation backcross (BC2; 12.5% domesticated alleles), first-generation backcross (BC1, 25% domesticated alleles), F1 hybrid (F1; 50% domesticated alleles) and pure domesticated (D; 100% domesticated alleles). Numbers to the left of sex symbols in the 2005 parents indicate the number of parents that were used overall, and numbers in brackets in the 2005 Offspring indicate the number of females fertilized as single-pair crosses (each female was fertilized with one male).