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P30His and P32His localize to the attachment organelle tip, the site of localization of native P30. Whole cells grown on glass coverslips (red channel) were probed with a monoclonal anti-6×His antibody and an FITC-conjugated secondary antibody (green channel). Strains are indicated in the upper left of each panel, except that NC indicates wild-type (WT) cells processed with only secondary antibody as a negative control. Bar, 2 µm.
