Table 1. Sex ratio in gametocyte cultures, determined by using various methods.

Male and female gametocytes were counted on smears of the following stages: stages IV and V for labelling of HA-Pfnek-1 transgenic parasites with antibodies against HA and Pfg377; stage V for Giemsa-stained gametocytes of the wild-type 3D7 clone; stages IV and V for labelling gametocytes of the wild-type 3D7 clone with antibodies against α-tubulin and Pfg377. See text for details.

	No. of female gametocytes	No. of male gametocytes	Sex ratio (%)*
Pfg377/HaNek1	155 (red)	8 (green)	5
Giemsa stain	117 (blue)	8 (pink)	6.4
Pfg377/Tubulin	235 (red)	21 (green)	8

^*Male/(male+female)×100.