Table 2. Oligonucleotides used in this study.

Functional restriction-enzyme sites added to the primer are underlined. The sequence for one copy of the bmpB terminator is indicated in bold. The sequence for the B. burgdorferi codon usage-optimized FLAG epitope is indicated in italics. The engineered ATG sequence in primer 5 is indicated in lower-case letters.

Number	Name	Sequence 5′–3′
1	bmpB termX3 FWD	CCCCCCCAATTGCTTTTTTGGCTGGCTATATTGCAGCCAAAAAAAGCTTTTCGCTTTTTTGGCTGGCTATATTGCAGCCAAAAAAAGCTTTTCGCTTTTTTGGCTGGCTATATTGCAGCCAAAAAAAGCTTTTCGGAATTCCG
2	bmpB termX3 REV	CGGAATTCCGAAAAGCTTTTTTTGGCTGCAATATAGCCAGCCAAAAAAGCGAAAAGCTTTTTTTGGCTGCAATATAGCCAGCCAAAAAAGCGAAAAGCTTTTTTTGGCTGCAATATAGCCAGCCAAAAAAGCAATTGGGGGGG
3	pncA 5′ EcoRI N	CGGAATTCCTATGCTATCCCCTTGTTCAAGTC
4	pncA FLAG XbaI 3′	GCTCTAGATTATTTATCATCATCATCTTTATAATCTATATTAAGCTTACTTTGGCTG
5	pncA 5′ EcoRI A	CGGAATTCatgGCACTTATTTTAATAGATATAC
6	pncA 5′ EcoRI B	CGGAATTCATGCCAGTATCTAACAGTAATG
7	flaB prom 5′ EcoRI	CGGAATTCTGTCTGTCGCCTCTTGTGGCTTCC
8	flaB prom 3′ EcoRI	CGGAATTCGATTGATAATCATATATCATTCC