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. Author manuscript; available in PMC: 2012 May 16.
Published in final edited form as: Free Radic Biol Med. 2010 Nov 12;50(1):139–147. doi: 10.1016/j.freeradbiomed.2010.10.693

Figure 2.

Figure 2

Representative autoradiographs of 32P-labeled DNA products resulting from auto-oxidation of dopamine (30 μM) and Cu2+ (30 μM)-mediated activation of dopamine (30 μM) and H2O2 (30 μM). Specific conditions are described in text. An identical DNA decomposition pattern following auto-oxidation and Cu(II)-mediated oxidation of dopamine was found and visualized with longer exposure times. Oxidation DNA products were resolved by two-directional polyethyleneimine (PEI)-cellulose TLC (D1 = 45 mM sodium phosphate, pH 5.8/1 M formic acid onto a 6 cm Whatman no. 17 paper wick; D2 = 100 mM sodium phosphate, pH 6.0/10% acetonitrile (v/v). OR, origin.