Figure 2.

Quantitative real-time PCR validating the expression differences between TG and WT lungs for selected genes targeted by miRNAs that were upregulated in TG mice. Genes chosen for validation study were Pdgfra (targeted by mmu-miR-34a) and Prkca (targeted by mmu-miR-362). Both of these genes were found to be downregulated in the TG lungs (p = 0.0006 and 0.0054, respectively), confirming the data from the gene expression array. The ΔCT values were calculated by subtracting the CT values of the endogenous control Gapdh from the CT values of the target genes.