Fig. 8.

Surface biotinylation demonstrates reduced ENaC expression after Rab11b knockdown. A: surface biotinylation was performed on filter-cultured mpkCCD cells transfected with control, Rab11a, or Rab11b siRNA. Apical surface expression of the 3 ENaC subunits is presented in the top blots, with an actin control to demonstrate the specificity of the biotin labeling. Whole-cell lysate (WCL) blots are presented at the bottom to probe for whole cell levels of ENaC and Rab11. B: quantification of band intensities for n > = 2 experiments are presented for all proteins. *Significant reduction (P < 0.01) in expression compared with control siRNA cells.