Table 2.
Mutations in hepatitis B virus (HBV) reverse-transcriptase genes detected by direct polymerase chain reaction (PCR) sequencing and ultra-deep pyrosequencing (UDPS) in nucleoside- and nucleotide-naive HBV-infected patients.
| Subject identifier | HBV genotype | Mutations detected by direct PCR | Additional mutations detected by UDPSa (sample reads detected, %) |
|---|---|---|---|
| D4 | B | N131D, N134I, R138K, N139H, T225T/I, S256C | R13H/L (3.3, 2.0), N53D (4.2), M98I (7.3), N118D (4.0), N123H (11),N124K (4.0), R138E (3.9), S219T (7.1), S256G (3.2), S332R (16),K333Q/H (11/2.4), L336M (2.7) |
| F1 | B | … | E11Q (3.3) |
| A7 | B | E1D, T54N, N134D, S332R | Y141F (2.9), M204I (1.3), P325L (10), A329T (11) |
| C2 | C | S78S/T, V191I/V, I269I/L, S317A, C332C/R, K333N, N337H | T38A (20), S53I/N/V (12/4.7/3.4), T54N (3.3), H55R (20), V214A/E (20/3.1), F221Y (2.0) |
| C7 | C | T38T/A, S53S/I, H55H/R, L267Q, Q271H | S53N/V (10/8), T54N (8.3) |
| D3 | A | A7T, R110R/G, N122H, Y126H, T259S, V266I | R120K (2.1), V191I (3.0) |
| E6 | B | E1E/D, R18R/K, L91L/I, N134D, Y141Y/F, A222A/T, S256G, K270K/R | R13H (5.9), T128N (6.6), N131D (6.4), N134E (7.1), A181T (1.0), M204I (1.0), S213T (5.2), A329S (11), S332R (9.0), K333Q (7.9), N337D (13) |
| G3 | B | N124H, N134D, S135S/Y, I187V, T259S, M271L, M309L | I233V (2.4), S332R (2.7) |
| B1 | B | N124D, N134N/D, N139N/D, S213T, N226H | N33S (5.4), L91I (4.8), R138K (2.0), S332R (8.5), N337H (3.8) |
| B5 | B | N124H, T128T/N, N134D, L220I/V, I233I/V, T259S, M271L | A329T (14.4), S332R (4.2) |
| 7774 | A | A7D, I103V, L129M, S143S/T, I53I/N/V/D, T54T/N, S116S/T, I163V, L217R, S219S/A, V253I | A7N (15), H35N (4.2), I53S (2.7), D134E (6.0), R138K (8.9), V207I (8.8), N238H (7.7), H271Q (4.3), M336L (10) |
| LYC | B | R274K, N337T | N33I (2.3) |
| JS | B | N124H, N134D, V207M, T259S, M271L, T259S, K333Q | N33I (2.5) |
| SH | C | D134D/E, L269I, K318R | … |
| GB1 | A | A7D, I53I/G/S/V, L129M, L132L/M, I163V, L217R, S219S/A, V253I | A329T (14) |
| LS | B | N134D, Y141Y/F, S213S/T | H9N (8.0), N33I (3.8), S256G (17) |
| LCS | B | N76N/D, S119S/T, N134D, Y141Y/F, L220I, S256C, Q316H | N33I (3.5), H35Q (18), T54N (4.5), L220V (2.1), I233V (4.9), Q319R (3.3), K333Q (12) |
NOTE. Mutations were defined as differences from the consensus genotype sequence. Mutations in bold type are established nucleoside and/or nucleotide reverse-transcriptase inhibitor (NRTI)–resistance mutations. Underlined mutations have been considered accessory NRTI-resistance mutations.
a
Mutations shown are those with a prevalence ≥1.0% at positions known to be associated with NRTI resistance or prevalence ≥2.0% at any other reverse transcriptase position.