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. 2011 Aug 25;21(8):1333–1343. doi: 10.1089/scd.2011.0139

FIG. 4.

FIG. 4.

hASCs and hBM-MSCs decrease NK cell activity through contact-dependent and independent mechanisms. Allogeneic NK cells sorted on the basis of CD56+CD3- phenotype were co-cultured at a ratio 1:1 in the presence or absence of hASCs or hBM-MSCs for 72 h in transwell or direct contact. These cells were subsequently tested in a degranulation assay against a NK-susceptible target cell line (K562 cells). The graph represents degranulation of natural killer cultured alone (NKcontrol), degranulation of NK cells preincubated with hASCs (NKhASCs), and degranulation of NK cells pre-incubated with hBM-MSCs (NKhBM-MSCs). Values represent the mean±SD of 4 independently performed experiments. NKhASCs, natural killer presensitized with hASCs; NKhBM-MSCs, natural killer presensitized with hBM-MSCs. *Significant at P≤0.05. #Nonsignificant at P≥0.05.