Skip to main content
. 2011 Aug 23;21(8):1299–1308. doi: 10.1089/scd.2011.0200

FIG. 1.

FIG. 1.

Purification of nonpericyte MSC-like cells from human adipose tissue. (a) After exclusion of DAPI+ dead cells and CD45+ hematopoietic cells, pericytes were isolated as CD146+CD34- cells (gray gate) as previously described. MSC-like cells could also be grown from the mixed population of nonpericyte cells differentially expressing CD34 and CD146 (black gate). (b) The following nonpericyte cell populations were then purified: CD34+CD146-, CD34+CD146+ and cultured in MSC conditions. The outgrowth of MSC-like cells was observed only after culture of CD34+CD146- cells. (c) Analysis of CD31 expression on gated CD34+CD146- cells (left dot plot) and CD34+CD146+ cells (right dot plot). CD31 was detected in only endothelial CD34+CD146+ cells, whereas no endothelial cells were detected within CD34+CD146- cells. (d) RT-PCR analysis was performed to check the purity of sorted CD34+CD31-CD146- cells. CD34 but no CD31, CD144, and CD146 mRNA was detected, thus excluding a possible contamination by endothelial cells or pericytes. C, positive control (unfractionated stromal vascular fraction); S, freshly sorted CD34+CD146- cells. The same results were observed in 3 distinct experiments. MSC, mesenchymal stem cell.