Table 1.
Accuracy of West Nile Virus Differentiation Algorithm As Determined by Serological Screening and Confirmatory Diagnostic Tests Using Reference Sera and Serum Samples Collected from Animals Experimentally Infected with West Nile Virus (WNV) and Japanese Encephalitis Virus (JEV)
| |
|
WNV |
JEV |
Anti-WNV antibody screening test |
Interpretation by PRNT and WNV differentiation algorithm |
WNV differentiation algorithm accuracy |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Species | No. of samples | Pos | Neg | Pos | Neg | MAC- ELISA positive | IgG ELISA positive | Negative | WNV | JEV | WNV negative | Specificity (%) | Sensitivity (%) |
| Horse | 7 | 4 | 3 | 4 | 4 | 3 | 4 | 0 | 3 | 100 | 100 | ||
| 21 | 13 | 8 | 1 | 7 | 13 | 13 | 8 | 100 | |||||
| Rabbit | 66 | 41 | 25 | nt | 41 | 25 | 41 | 0 | 25 | 100 | 100 | ||
| 47 | 47 | nt | 37 | 10 | 0 | 37 | 10 | 100 | |||||
| Chicken | 50 | 30 | 20 | 26 | 29 | 20 | 30 | 0 | 20 | 100 | 100 | ||
| 27 | 27 | 21 | 19 | 6 | 0 | 21 | 6 | 100 | |||||
Equine reference serum samples obtained from the Reference Laboratory of the World Animal Health Organization (Office International des Epizooties) at the National Veterinary Services Laboratories, United States Department of Agriculture, Ames, Iowa.
IgG, immunoglobulin G; ELISA, enzyme-linked immunosorbent assay; PRNT, plaque reduction neutralization test; MAC-ELISA, immunoglobulin M antibody capture ELISA; Pos, positive; Neg, negative; nt, not tested.